Functional responsiveness of in vitro-aged human neutrophils

Auteurs

J. Morin-Genest, Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, Institut National de la Recherche Scientifique (INRS), Centre Armand-Frappier Santé Biotechnologie, Laval, QC, Canada
A. Saafane, Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, Institut National de la Recherche Scientifique (INRS), Centre Armand-Frappier Santé Biotechnologie, Laval, QC, Canada
D. Girard, Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, Institut National de la Recherche Scientifique (INRS), Centre Armand-Frappier Santé Biotechnologie, Laval, QC, Canada

Type de document

Études primaires

Année de publication

2023

Langue

Anglais

Titre de la revue

Cellular Immunology

Résumé

Elimination of apoptotic neutrophils by macrophages is as a major step for the resolution of inflammation. However, the fate and the cellular functionality of neutrophils aged in the absence of macrophages are not well documented. Herein, freshly isolated human neutrophils were aged for several days in vitro and then stimulated with agonists for determining their cell responsiveness. In vitro-aged neutrophils were still able to generate reactive oxygen species after 48 h, exert phagocytosis after 72 h, and increase their adhesion onto a cell substratum after 48 h. These data demonstrate that a portion of neutrophils cultivated for several days in vitro are still able to exert biological functions. This opens the possibility that, during inflammation, neutrophils may still respond to agonists, a condition that is likely to occur in vivo when they are not efficiently eliminated by efferocytosis. © 2023 Elsevier Inc.

Mots-clés

Altération des neutrophiles, Neutrophil change, Recherche in vitro, In vitro experiment

Numéro de projet IRSST

2017-0044

Citation recommandée

Morin-Genest, J., Saafane, A. et Girard, D. (2023). Functional responsiveness of in vitro-aged human neutrophils. Cellular Immunology, 390, article 104739. https://doi.org/10.1016/j.cellimm.2023.104739