High-sensitivity permeation analysis of ultrasmall nanoparticles across the skin by positron emission tomography

Auteurs

Mahmoud M. Omar, Département de Génie des Mines, de la Métallurgie et des Matériaux, Centre de Recherche sur les Matériaux Avancés (CERMA), Université Laval, Québec, G1V 0A6, Canada, Axe Médecine Régénératrice, Centre Hospitalier Universitaire (CHU) de Québec, 2705, boul. Laurier (T1-61a), Québec, G1V 4G2, Canada
Myriam Laprise-Pelletier, Axe Médecine Régénératrice, Centre Hospitalier Universitaire (CHU) de Québec, 2705, boul. Laurier (T1-61a), Québec, G1V 4G2, Canada
Pascale Chevallier, Axe Médecine Régénératrice, Centre Hospitalier Universitaire (CHU) de Québec, 2705, boul. Laurier (T1-61a), Québec, G1V 4G2, Canada
Ludovic Tuduri, Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherche 5805, Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Équipe Physico et Toxico Chimie de l'Environnement, Université de Bordeaux, Talence, 33405, France
Marc-André Fortin, Département de Génie des Mines, de la Métallurgie et des Matériaux, Centre de Recherche sur les Matériaux Avancés (CERMA), Université Laval, Québec, G1V 0A6, Canada, Axe Médecine Régénératrice, Centre Hospitalier Universitaire (CHU) de Québec, 2705, boul. Laurier (T1-61a), Québec, G1V 4G2, Canada

Type de document

Études primaires

Année de publication

2021

Langue

Anglais

Titre de la revue

Bioconjugate Chemistry

Première page

729

Dernière page

745

Résumé

Ultrasmall nanoparticles (US-NPs; <20 nm in hydrodynamic size) are now included in a variety of pharmacological and cosmetic products, and new technologies are needed to detect at high sensitivity the passage of small doses of these products across biological barriers such as the skin. In this work, a diffusion cell adapted to positron emission tomography (PET), a highly sensitive imaging technology, was developed to measure the passage of gold NPs (AuNPs) in skin samples in continuous mode. US-AuNPs (3.2 nm diam.; TEM) were functionalized with deferoxamine (DFO) and radiolabeled with 89Zr(IV) (half-life: 3.3 days, matching the timeline of diffusion tests). The physicochemical properties of the functionalized US-AuNPs (US-AuNPs-PEG-DFO) were characterized by FTIR (DFO grafting; hydroxamate peaks: 1629.0 cm-1, 1569.0 cm-1), XPS (presence of the OC-N C 1s peak of DFO at 287.49 eV), and TGA (organic mass fraction). The passage of US-AuNPs-PEG-DFO-89Zr(IV) in skin samples was measured by PET, and the diffusion parameters were extracted thereby. The signals of radioactive US-AuNPs-PEG-DFO-89Zr(IV) leaving the donor compartment, passing through the skin, and entering the acceptor compartment were detected in continuous at concentrations as low as 2.2 nM of Au. The high-sensitivity acquisitions performed in continuous allowed for the first time to extract the lag time to the start of permeation, the lag time to start of the steady state, the diffusion coefficients, and the influx data for AuNPs permeating into the skin. PET could represent a highly valuable tool for the development of nanoparticle-containing topical formulations of drugs and cosmetics. © 2021 American Chemical Society.

Mots-clés

Nanoparticule, Nanoparticle, Pharmacologie, Pharmacology, Contamination de la peau, Skin contamination, Or, Gold, CAS 7440575, Tomographie, Tomography

Numéro de projet IRSST

2015-0084

Citation recommandée

Omar, M. M., Laprise-Pelletier, M., Chevallier, P., Tuduri, L. et Fortin, M.-A. (2021). High-sensitivity permeation analysis of ultrasmall nanoparticles across the skin by positron emission tomography. Bioconjugate Chemistry, 32(4), 729-745. https://doi.org/10.1021/acs.bioconjchem.1c00017