Bioaerosol sampler choice should consider efficiency and ability of samplers to cover microbial diversity

Auteurs

Hamza Mbareche, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Quebec City, QC, Canada, Département de Biochimie, De Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Quebec City, QC, Canada
Marc Veillette, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Quebec City, QC, Canada
Guillaume J. Bilodeau, Pathogen Identification Research Lab, Canadian Food Inspection Agency (CFIA), Ottawa, ON, Canada
Caroline Duchaine, Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Quebec City, QC, Canada, Département de Biochimie, De Microbiologie et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Quebec City, QC, Canada

Type de document

Études primaires

Année de publication

2018

Langue

Anglais

Titre de la revue

Applied and Environmental Microbiology

Résumé

Bioaerosol studies aim to describe the microbial content and increase understanding of the aerosolization processes linked to diseases. Air samplers are used to collect, identify, and quantify bioaerosols. Studies comparing the performances of air samplers have typically used a culture approach or have targeted a specific microorganism in laboratory settings. The objective of this study was to use environmental field samples to compare the efficiencies of 3 high-airflow-rate samplers for describing bioaerosol diversity using a next-generation sequencing approach. Two liquid cyclonic impactors and one electrostatic filter dry sampler were used in four wastewater treatment plants to target bacterial diversity and in five dairy farms to target fungal diversity. The dry electrostatic sampler was consistently more powerful in collecting more fungal and bacterial operational taxonomic units (OTUs). Substantial differences in OTU abundances between liquid and dry sampling were revealed. The majority of the diversity revealed by dry electrostatic sampling was not identified using the cyclonic liquid impactors. The findings from this work suggest that the choice of a bioaerosol sampler should include information about the efficiency and ability of samplers to cover microbial diversity. Although these results suggest that electrostatic filters result in better coverage of the microbial diversity among the tested air samplers, further studies are needed to confirm this hypothesis. While it is difficult to determine a single universally optimal air sampler, this work provides an in-depth look at some of the considerations that are essential when choosing an air sampler for studying the microbial ecology of bioaerosols. © 2018 American Society for Microbiology.

Mots-clés

Aérosol, Aerosol, Pollution microbienne, Microbial pollution, Échantillonneur aérosol électrostatique, Electrostatic aerosol sampler, Échantillonnage dans l'air, Air sampling, Essai du matériel, Equipment testing

Numéro de projet IRSST

2010-0050

Citation recommandée

Mbareche, H., Veillette, M., Bilodeau, G. J. et Duchaine, C. (2018). Bioaerosol sampler choice should consider efficiency and ability of samplers to cover microbial diversity. Applied and Environmental Microbiology, 84(23). https://doi.org/10.1128/AEM.01589-18