Organic components of airborne dust influence the magnitude and kinetics of dendritic cell activation

Auteurs

Magali Boucher, Centre de recherche, l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC, Canada
Pascale Blais Lecours, Centre de recherche, l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC, Canada
Valérie Létourneau, Centre de recherche, l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC, Canada
Marc Veillette, Centre de recherche, l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC, Canada
Caroline Duchaine, Centre de recherche, l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC, Canada, Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec, QC, Canada
David Marsolais, Centre de recherche, l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 chemin Sainte-Foy, Québec, QC, Canada, Département de médecine, Faculté de médecine, Université Laval, Québec, QC, Canada

Type de document

Études primaires

Année de publication

2018

Langue

Anglais

Titre de la revue

Toxicology in Vitro

Première page

391

Dernière page

398

Résumé

Bioaerosol exposure in highly contaminated occupational settings is associated with an increased risk of disease. Yet, few determinants allow for accurate prediction of the immunopathogenic potential of complex bioaerosols. Since dendritic cells are instrumental to the initiation of immunopathological reactions, we studied how dendritic cell activation was modified in response to individual agents, combined microbial agents, or air sample eluates from highly contaminated environmental settings. We found that combinations of agents accelerated and enhanced the activation of in vitro-generated murine bone marrow-derived dendritic cell cultures, when compared to individual agents. We also determined that endotoxins are not sufficient to predict the potential of air samples to induce bone marrow-derived dendritic cell activation, especially when endotoxin levels are low. Importantly, bone marrow-derived dendritic cell activation stratified samples from three environmental settings (swine barns, dairy barns, and wastewater treatment plants) according to their air quality status. As a whole, these results support the notion that the interplay between bioaerosol components impacts on their ability to activate dendritic cells and that bone marrow-derived dendritic cell cultures are promising tools to study the immunomodulatory impact of air samples and their components. © 2018 Elsevier Ltd

Mots-clés

Composés organiques, Organic compounds, Poussière en suspension dans l'air, Airborne dust, Aérosol, Aerosol, Endotoxine, Endotoxin, Culture de cellules, Cell culture, Échantillonnage dans l'air, Air sampling, Recherche in vitro, In vitro experiment

Numéro de projet IRSST

2010-0061

Citation recommandée

Boucher, M., Blais Lecours, P., Létourneau, V., Veillette, M., Duchaine, C. et Marsolais, D. (2018). Organic components of airborne dust influence the magnitude and kinetics of dendritic cell activation. Toxicology in Vitro, 50, 391-398. https://doi.org/10.1016/j.tiv.2018.04.011