Type de document
Études primaires
Année de publication
2022
Langue
Anglais
Titre de la revue
Rapid Communications in Mass Spectrometry
Résumé
Rationale
Toluene diisocyanate (TDI) is a highly reactive isocyanate commonly used as a mixture of 2,4- and 2,6- isomers in the production of flexible foams. Exposure to TDI occurs primarily through vapour inhalation in workplaces where TDI is produced or used, but dermal exposure is also possible during some tasks. To ensure workplace safety, accurate monitoring of TDI and toluene diamine (TDA) levels is required. Methods of quantifying field effectiveness of gloves in preventing dermal exposure have not been established. Therefore, there is a need to develop a new practical method for assessing glove effectiveness for TDI/TDA.
Method
A new offline SPE UPLC–MS/MS method for the quantitation of TDA isomers from TDI-exposed gloves was developed. Gloves were dipped in a solution of 1% acetic acid leading to a full conversion to TDA. TDA-free amine compounds were derivatized with acetic anhydride to increase chromatographic retention and signal intensity.
Results
2,4-Diaminotoluene-α, α, α-d3 (2,4-d3-TDA) was selected as a surrogate standard to minimise the variability in sample preparation and instrumental sensitivity. The choice of UPLC–MS/MS operated in multiple reaction monitoring (MRM) mode allowed to reach much lower limits of detection (LOD). The LOD of the method was 6.86 and 2.83 ng/mL (0.03 and 0.01 μg) for 2,6-TDA and 2,4-TDA, respectively. The limit of quantitation (LOQ) was 22.85 and 9.42 ng/mL (0.11 and 0.05 μg) for 2,6-TDA and 2,4-TDA, respectively.
Conclusion
A new UPLC–MS/MS analytical method has been developed to determine field effectiveness of gloves for preventing dermal exposure to TDI/TDA. The new technique overcomes some limitations for measuring putative dermal exposure to isocyanates and may be useful in exposure monitoring and future research on isocyanate health risks.
Mots-clés
Diisocyanate de toluène/mélange d'isomères, Toluene diisocyanate/mixture/, CAS 26471625, Évaluation de l'exposition, Exposure evaluation, Essai du matériel, Equipment testing, Gant de protection, Protective glove
Numéro de projet IRSST
n/a
Citation recommandée
Daoud, A., Cloutier, P.-L., Gagné, S., Breau, L. et Lesage, J. (2022). Development of a new SPE UPLC–MS/MS method for extraction and quantitation of toluene diamine on gloves following toluene diisocyanate exposure. Rapid Communications in Mass Spectrometry, 36(16), article e9340. https://doi.org/10.1002/rcm.9340
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