In vivo proinflammatory activity of generations 0-3 (G0-G3) polyamidoamine (PAMAM) nanoparticles
Type de document
Études primaires
Année de publication
2016
Langue
Anglais
Titre de la revue
Inflammation Research
Première page
745
Dernière page
755
Résumé
Objective and design
The aim of this study was to determine whether different generations (G) polyamidoamine (PAMAM) dendrimers possess proinflammatory activities in vivo.
Material or subjects
Several hundred female CD-1 mice were used to test four different PAMAM dendrimers using the murine air pouch model.
Treatment
Mice received appropriate negative and positive controls or G0-G3 PAMAM nanoparticles at 100 and 500 µg/ml into air pouches.
Methods
Exudates were harvested after 3, 6, 24 and 48 h. Cell pellets and supernatants were used to determine the number of total leukocytes and neutrophils and to detect the production of several analytes by an antibody array approach, respectively. One-way analysis of variance was used for statistical analysis.
Results
PAMAM dendrimers rapidly increased a leukocyte influx after 3 h, the vast majority of cells being neutrophils. This was also observed after 6 and 24 h, and resolution of inflammation was noted after 48 h. In general, the increased production of a greater number of analytes detected in the exudates after 6 h correlated with the number of dendrimer generations (G3 > G2 > G1 > G0).
Conclusions
PAMAM dendrimers devoid of any delivering molecules possess proinflammatory activities in vivo by themselves, probably via the production of different chemokines released by air pouch lining cells.
Mots-clés
Polyamides, Inflammation, Nanoparticule, Nanoparticle, Recherche sur l'animal, Animal experiment, Leucocyte, Leukocyte
Numéro de projet IRSST
2010-0021
Citation recommandée
Durocher, I. et Girard, D. (2016). In vivo proinflammatory activity of generations 0–3 (G0–G3) polyamidoamine (PAMAM) nanoparticles. Inflammation Research, 65(9), 745-755. https://doi.org/10.1007/s00011-016-0959-5